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It is also possible to use already processed data to classify examples as yet unseen.
In such cases we speak about data streams, and specific algorithms are needed to analyze progressively the data, store in memory only a small number of summary statistics, and then discard the already processed data and free the memory [7].
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In the following sections and in Additional file 2 we describe the bioinformatics pipeline that we used to analyze our small RNA deep-sequencing data and the already processed validation data from the TCGA.
Most existing Proteomics databases are usually related to only one type of data and/or represent already processed results, not raw data.
The results were validated on about 600 independent samples: experimentally by qRT-PCR on normal samples, tumors and LNM from 14 independent patients and in silico using the data from The Cancer Genome Atlas (small RNA deep-sequencing data already processed of 495 PTC, 59 normal samples and 8 LNM) [ 30].
The TCGA gene expression data is already processed and normalized.
For these ones, data were already processed by the providers and the tumor purity values were available, allowing us to perform the correction.
One benefit of using association rules is that it is very quick and easy to obtain relationships between various chemicals and heath markers because the data are already processed.
So don't panic – take it as an opportunity to review how you process data already and make sure you've got plans in place to make any changes that you need to be ready for next May.
We log2 transformed and quantile normalized gene expression signal intensities within each data set, if not already processed as such.
if you've already pre-processed your data, then you may only need steps 5,6,7 … please provide the –steps flag as needed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com