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Thus, complemented Δ ccm1 segregants were subjected to SMDC by: (i) repressing Ccm1p expression with dextrose (t=0) and (ii) en route constraining mitochondrial import of Ccm1p by fusing two synthetic IgG-binding domains of SPA (ZZ) to the C-terminus of CCM1 ORF (pCCM1ZZLC), thus delaying the import of the remaining protein whose expression has already been repressed.
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Indeed, the expression of many genes such as SUC2 is already known to be repressed by both Mig1 and Mig2 in glucose media, although Mig1 is the main factor in this response [ 20, 26].
The HGF mRNA was repressed already after 1 day of DOX treatment (Figure 1F), indicating effective reversibility of the Tet-Off system.
"These people already self-pityingly style themselves as a silent majority whose opinions are repressed by the state," he wrote at the Left Foot Forward Web site.
Even religion was repressed".
Mine is repressed.
I am repressed.
The 1956 Hungarian Revolution was repressed.
"Maybe it's repressed sexuality.
"He's repressed it all.
Demonstrations were repressed with heavy force.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com