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There is however, no experimental evidence available in the literature if the LC catalytic domain enters the cytosol and functions alone, dissociated from the rest of the molecule.
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In a small subset of the cases, however, the diVCA component alone dissociates, and this almost invariably leads to branch formation.
Yet it is unfortunate that not a single study has attempted to replicate these findings in children exposed to PCBs or Pb, let alone dissociate such putative effects from confounding contaminants, such as methylmercury (MeHg).
In contrast to oligomeric constructs, we show that WRN helicase alone can completely dissociate the 84 bp duplex to release the invading strand from the plasmid D-loop.
In contrast, expression of the mCherry-MVP mRNA alone resulted in unstable vaults that rapidly dissociated into halves under conditions used for uranyl acetate staining.
In contrast to wild type SV40, which required both DTT and EGTA for disassociation, VLPs consisting of VP1, VP2 and VP3 completely dissociated by incubation with either DTT or EGTA alone [37].
To generalize our findings beyond the setting of established NSCLC-SC cell lines, we evaluated the colony-forming ability of freshly dissociated NSCLC cells in the presence of chemotherapy alone or in combination with AZD7762.
To assess the effects of INHBA overexpression in hAMSCs, we assembled cultures of PHBECs alone or with either control or INHBA-overexpressing hAMSCs (hAMSC INHBA), dissociated the cultures after 30 days, and subjected single cells to colony formation assays.
To control for DR1 binding to freshly dissociated peptides on the chip, a second injection of wtDR1/HAY308A alone was performed on a control flow cell.
For the REMD simulation of holo-LFABP bound with two molecules of the ligands (OLA or 1,8-ANS), the random acceleration, unless otherwise stated, was firstly applied to the ligand at the low affinity site alone, and shifted to the another ligand after the first one had dissociated away from the protein.
Clonally-derived tumorspheres (LA-N-5, IMR-32, CHP-134) were dissociated, plated on chamber-slides and grown in media containing serum alone or supplemented with neurogenic, gliogenic or smooth muscle fibroblastic growth factors.
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