The phrase "alone bound to" is not standard in written English and may cause confusion.
It could be used in contexts discussing obligations or commitments that are solely one's own, but it is not commonly used.
Example: "In this situation, I feel alone bound to make the right decision for myself."
Alternatives: "solely obligated to" or "exclusively tied to".
GATA2-Zf alone bound to TSH GATA-RE with a Kd of 688+/−39 nM (n = 3.1).
For this reason, all experiments were carried out on cultures prior to passage 6. AP-binding assays indicated that msPlxdc2-AP but not AP alone bound to ENCs (Fig. 11, c and d).
A dashed line indicates the position of the clamp alone bound to a DNA substrate.
The eluted His-tagged proteins were incubated with purified GST-PID2K or GST alone bound to glutathione beads, respectively.
In a similar manner, mice immunized with his-p24/his-Nef/ Ni-NP had greater Nef-specific antibodies than mice immunized with Nef alone bound to NPs.
Western blotting showed that GST-VAMP7 longin domain, but not GST-Sec22b longin domain nor GST alone, bound to endogenous Hrb.
Furthermore, Dkk1 alone binds to LRP6 and initiates LRP6 internalization [32], [33], which was confirmed in this study, showing that Dkk1 treatment increases LRP6 levels in endosomes.
A recent study by Stumhofer and colleagues [ 51] showed that IL-27 p28 alone binds to gp130 as a pro-inflammatory signal antagonist.
The reason for this discrepancy is that while NFAT can alone bind to and activate the CD40L promoter, binding to the IL2 promoter requires AP1 binding to adjacent sites.
With the DRA Y-box sequence as probe (Fig. 5b, lanes 6 10), the unlabelled probe sequence competes less well than do any of the other Y-box sequences, suggesting that rDEK alone binds to this sequence nonspecifically and with low affinity.
Accordingly, the movement (RMSD: 0.67 Å) of LPP-5 is slightly larger than that of LPP-3 (RMSD: 0.43 Å). 10.7554/eLife.07901.007 Figure 2. SAM alone binds to MT-2 of TP and triggers global movement of all capsid proteins.
