The experiment was designed to measure the inorganic carbon levels in three conditions: control (nutrient broth only), bacteria alone and bacteria with their specific phage.
McEnery and Lee (1976) previously demonstrated that bacteria alone are a poor food source for A. laticollaris CSH as cultures fed only bacteria either died or grew more slowly than algae-fed cultures.
Open image in new window Fig. 3 The growth rate of the studied bacterivorous ciliates when cultured alone without bacteria (heavy gray colored histogram), feeding on (E. coli, and S. typhi) mixture (light gray colored histogram) or ingesting of (E. coli and Sh. Flexneri) a mixture (white colored histogram).
Within the Riesia lineage alone, these bacteria undergo two extra-cellular migrations and are housed in no fewer than four distinct mycetomes [42].
The control construct to express the His-tagged HaloTag alone in bacteria is pGH494: The HaloTag coding sequence was amplified (oGH1166 + oGH1171) and recombined with the His-tag, T7 promoter and backbone regions of the AP2 mu domain construct (pGH441 amplified with oGH1170 + oGH1165).
Gram-positive bacteria alone, Gram-negative bacteria alone, and yeast alone were isolated from 210 (86%), 23 (9%), and six (3%) of these 244 samples, respectively.
The reduction in DNA synthesis observed here in bacteria-fed cultures suggests that bacteria alone are a poor food source.
The effect of rubbing in 1.6 g water alone reduced test bacteria by 2.4 log10 but this result might be because some bacteria were removed by the baseline sampling before water application, and some were removed by the 5 s rinse after application and before sampling.
Bacteria alone were isolated in 8.2% (367 of 4485), parasites alone in 5.6% (253 of 4485) and both parasites and bacteria in 0.5% (24 of 4485).
Certain bacteria alone, such as Azotobacter chroococcum and Clostridium pasteurianum, are also capable of fixing nitrogen.
