The simplest and most used of such methods assigns each read to its best matching reference sequence, called best hit (BH); as recently shown for instance in Brady and Salzberg (2009), this is still the best stand-alone assignment method for long reads (of length >800 bp).
Results obtained with SPANNER were compared with those obtained using only the LCA algorithm, assignments based on best BLAST alone and assignments using SOrt-ITEMS and CARMA3.
Further, using a high quality BLAST alignment alone for the assignment gives this approach the ability to also assign a gene that lies in a gap in the human genome sequence.
Then a teacher walks in and bursts into outrage: Max is supposed to be doing his assignment alone — the standard punishment, it would seem, for untoward tendencies.
How am I ever going to make it through this assignment alone?
To reveal information inaccessible in interviews alone, a photography assignment and second interview using photo-elicitation were used.
Based on fragment L alone, unambiguous lineage assignment was also possible for the 20 specimens for which all three fragments could not be gained.
In terms of domain assignment alone, a total of 3566 proteins (89%) could be associated with at least one structural or functional domain.
Unfortunately, we were unable to retrospectively study how molecular vs clinical risk assignment alone influences the multidisciplinary team (MDT) decision concerning the recommendation to administer adjuvant CHT.
Group assignment alone did not have an effect on test scores (p > 0.84), but intervention-arm subjects who completed all assigned cases answered an average of 3.2 more questions correctly on the post-test than others, controlling for pre-test scores (p = 0.003).
And even if the new boss leaves the assignments alone, departures have opened the trophy case.
