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Individual full length VirB6 genes are conserved across 13 genomes and range in nt divergence from 10.3% (12.4% aa) to 13.2% (15.2% aa); however, the five duplicates are extraordinarily different from one another, averaging ∼80% aa divergence in the VirB6/TrbL domains alone (alignment of full length proteins was confounded by variability in the flanking sequences).
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This was also supported by combined indel data from ancestral sequence reconstruction as well as by the data derived from alignments alone (alignments R = 0.23, ancestral sequences R = 0.33).
Five parameters are intrinsic to our split-read analysis alone: the alignment score ratio threshold tr, the threshold on the number of supportive reads for 1-bp SVs tn, the threshold on the maximum centeredness for large SVs tc, the minimum number of supportive reads for every SV identification nmin, and the exponential decay parameter λ.
Tew and Waugh found that properly aligned tibial components are the most successful, but proper alignment alone does not guarantee long-term success.
This change is larger than would be anticipated from enhanced calcium handling and increased uniaxial alignment alone.
However, optical momentum alignment alone is not enough to induce a net electric current due to the symmetrical distribution of carriers with momenta k and −k in optical excitation.
The coverage of MIPS was more than twice that of using sequence-based alignment alone.
Thus, the functions of these proteins cannot be annotated by sequence alignment alone.
Note that the speed of KalignP for alignment alone is comparable to Kalign2.
We also discriminate several common carp genes and gene isoforms that were not discriminated by BLAST sequence alignment alone.
The risk for revision noted in the present study for lateral release is likely separate from that associated with having valgus alignment alone, since valgus alignment was not associated with revision risk in our analyses.
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