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More recently, a transaminase showing almost no activity for a commercially interesting substrate was mutated to a highly active and selective catalyst enabling a new efficient industrial process for sitagliptin production (12).
While the LIPESV12_24 was still active at pH 5.5, the LIPESV12_24 at that pH had almost no activity.
These experiments confirmed that wild-type MLL3 is more active than Y4884C on the unmethylated peptide substrate, but it has almost no activity on mono- or dimethylated substrates.
"The zombie brain has almost no activity in the frontal lobe," he explains helpfully.
A A year and a half ago it was terrible — almost no activity.
"I can tell you right now, we've had almost no activity in TriBeCa," said Clark P. Halstead, president of the Halstead Property Company.
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Despite high HCO3−/Cl− exchange activity before meiotic maturation and in 1-cell embryos after fertilization, MII eggs surprisingly exhibit almost no HCO3−/Cl− exchanger activity [10], [11], [28].
The men, most under 35, have almost no activities to keep them occupied.
But there is also a growing number of families where children are being raised by others and sharing almost no activities with their parents.
In the case of NIK1, we further demonstrated that the correct position of the introduced carboxylate within the A-loop was relevant for activity because there was almost no detectable activity with the T474E mutant.
However, xylanase Xyn10A almost had no activity on CMC-Na.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com