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Almost complete assembly was achieved by primer walking, and assisted by detailed fosmid maps of the genomes.
454 sequencing provided a much more even coverage of the BAC clones than ABI-Sanger sequencing, resulting in almost complete assembly of all genic sequences even at only 9 to 10-fold coverage.
Wicker et al. (2006) suggested that 454 sequencing technology could reveal almost complete assembly of the entire gene sequences in 4 barley BAC clones at only 9-folds coverage and concluded that the method is a rapid and cost effective way of sequencing the gene-containing portions of the genome.
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We also mapped 10,748 EST sequences generated from our Ae. tauschii full-length cDNA libraries16 to the assembly; 10,471 (97.4%) of these could be mapped to the scaffolds with greater than 90% coverage, which indicated that gene regions were almost complete in our assembly.
Although this procedure was reliable for annotating human pseudogenes, presuming that the human reference genome assembly is almost complete and does not contain any sequencing and assembly errors, annotating pseudogenes in other species was often more difficult.
The zebrafish genome is being sequenced and with the release of Zv8 [17], the zebrafish genome assembly is almost complete.
The encapsidation process occurred at the replication centre periphery once capsid assembly is almost complete (Figure 3A).
With the assembly of the (almost) complete genome of the C57BL/6J strain, the ability to relate sequence to function was significantly enhanced (1, 2).
If the de novo assembly derives from high depth data sets, assembling them can produce almost complete genomes of high quality even from rare species [ 113, 114].
The new IAEA report notes that "the installation of cooling and moderator circuit piping was almost complete" at the Arak reactor, and that a prototype fuel assembly intended for the reactor was being subject to irradiation testing at the Tehran Research Reactor.
A gel-like suspension of fibrils formed almost immediately, and samples were incubated at 25 °C for 7−10 days to ensure complete assembly prior to biophysical characterization.
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