A cross-analysis of a rationally designed panel of RNA binding and ATPase mutants and truncated receptors, using type I IFN promoter activation as readout, allows us to refine our understanding of the structure-function relationships of RIG-I and MDA5.
We propose several coding schemes and applications: (1) coded readout allows us to better sample time dimension for high-speed photography and optical flow based applications, including motion interpolation, skew compensation, and motion deblur; and (2) row-wise control enables capturing motion-blur free high dynamic range images from a single shot.
In contrast, optical readout of the ionic current allows us to trace every change in the electrical current to a specific pore.
This HSR readout in our zebrafish model allows us to track the axons of stressed motor neurons into the muscle and evaluate whether stress in the motor neuron perikaryon leads to detectable pathology at the NMJ.
What allows us to survive?
The transparent disk treadmill allowed us to improve readout of various aspects of cerebellum-dependent motor behavior including gait, posture, and small limb movements, advancing upon previous treadmill systems that also permit concurrent readout of neural circuits and behavioral state [ 32], but not more detailed aspects of complex movements.
We used a fast CCD camera that allowed us to compress the readout process to several milliseconds.
This would allow us to use it as a rapid readout of bacterial numbers as an alternative to the lengthy colony counts (taking 3 4 weeks).
Gaseous concentrations and PID voltage readings followed a linear relationship, which allowed us to convert the PID readout into absolute concentration.
The ability to detect a clear phenotypic readout that could distinguish between BRAFWT and BRAF disease alleles, allowed us to use the zebrafish as the basis for further investigation of the CFC-pathway allele function.
