Syncytial embryos are amenable to time-lapse imaging of hundreds of synchronously dividing spindles, allowing the quantitation of spindle and chromosome dynamics with unprecedented fidelity.
This is the first assay to allow the quantitation of NQO1 in live cells which can then be retained for further experiments.
This assessment allowed the quantitation of mRNA changes due to microRNA regulation of Ad5mir122 but also served to highlight any mRNA differences between Ad5mir122 and Ad5WT.
Even the use of in vivo cytotoxicity (IVC) assays, [18] which allow the quantitation of T cell cytolytic activity in spleens and lymph nodes (LNs) of tumor-bearing mice, [19], [20] does not address the impact of the tumor site itself.
Finally, BSAS does not allow the quantitation of both 5-mC and 5-hmC.
Forward mutational analyses allow the quantitation of mutation rates in the artificial microsatellites after vector isolation and genetic selection in E. coli.
It is based on scanning for intensely fluorescent targets (SIFT), a unique technique allowing the quantitation of de novo protein aggregation in solution [ 26].
All images were processed using the TissueQuest software (TissueGnostics), allowing the quantitation of the total cell numbers from DAPI positive cells, as well as quantitation of target-positive cells.
The combination of multiple NMR experiments allowed the quantitation of the fate of labeled glucose carbon in proteins, peptides, intracellular sugars and glycerol, and lipids, yielding values of 9, 2, 7, and 4%, respectively (Table 1).
The deconvolution of the DAB channel allowed the quantitation of IHC staining for each of the four IHC stains within each ROI, defined by the average optical density of DAB times the percent of area staining positive with DAB.
