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Hence, the zebrafish Danio rerio is gaining popularity in metabolic research and drug discovery, as this animal model allows screening of small molecules in the context of the whole-organism.
We obtained functional switches from a pool of variants carrying randomized connection sequences by an in vivo selection in MaV203 yeast cells that allows screening of a large sequence space of up to 1 × 109 variants.
Furthermore, the use the 384 well format allows screening of large libraries using a relatively small amount of parasite lysates.
The physical linkage between the displayed protein and the DNA encoding it allows screening of vast numbers of proteins for ligand-binding properties [36].
After completing many iterations of this process, involving the dissection and staining of embryos from more than 700 Df lines, we were able to define a new kit of 423 lines, which allows screening of 80 90% of the genome for Lar-AP staining, or staining with other reagents that recognize CNS axons.
Transfected cell microarrays [8] [11], where cells are grown over a glass slide printed with transfection constructs, allows screening of expression [11] or RNA interference libraries [9] for functional genomics or drug screening [8], [10], and the technology allows multiple conditions to be tested on replicate slides.
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Designed pharmacophore models allowed screening of 3D structural domains.
We adapted this assay to allow screening of mutant libraries of either l- or d-amino acid specific aminotransferases in a continuous fashion.
DNA microarrays currently allow screening of the entire human genome with high level of coverage and we are now entering the era of high-throughput sequencing.
The load-balancing, fault-tolerance, and scalability in MapReduce allow screening of very large conformation spaces not approachable with traditional clustering methods.
In principle, efficacy of biomaterial development for the cell cultures can be improved by using high-throughput techniques that allow screening of a large number of materials and manipulate microenvironments in a controlled manner.
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