These results do not rule out any specific model for the mechanism by which miRNAs repress translation in the ovary, but do allow for translational inhibitory mechanisms that act after initiation of translation.
The resulting PCR fragments were digested with EcoRI and BamHI and ligated into pCK-lac, a derivative of pTCV-lac containing a lacZ gene without a Shine-Dalgarno sequence or start codon allowing for translational analysis of the gene in question.
CHOP and GADD34 mRNAs also contain uORFs that allow for translational regulation upon eIF2α phosphorylation (Lee et al., 2009; Palam et al., 2011).
Furthermore, an additional construct was generated in such a way that it contained the whole native 5′UTR sequence together with the sequence encoding the putative transit peptide, allowing for translational initiation at potential non-AUG start codons and to rule out GFP localisation due to forced translational initiation (Kabeya and Sato 2005).
The thin coplanar nanometer-scale stage allows for three-translational and two-rotational motions and is provided with eight piezoelectric actuators one on the X-axis, another on the Y-axis, and the others on the Z-axis.
Rigid image transformation only allows for rotational and translational movements of the entire image, whereas non-rigid image registration allows for any type of local (elastic) deformations.
A second difference that might apply to this discussion is that the localization of Twinkle variants in mitochondria allows for potential post-translational modification that may influence and regulate the activity of Twinkle in vivo.
Furthermore, there is experimental evidence of a synergistic effect between multiple miRNP complexes associated with a single mRNA [ 21], suggesting that multiple target sites may allow for rapid translational control [ 32]. 2) Strength of miRNA-mRNA hybridization.
This same idea has been proposed in polarized cells, where the coupling of mRNA retention and local translational allows for efficient sorting to the final sites of membrane deposition and/or secretion (Herpers and Rabouille, 2004).
Additionally, this assignment method allowed for post-translational modifications reported in the NCBI database and has previously been utilized [24].
