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The latter technique allows for profiling of RNA sequences by deep sequencing that are protected from degradation by associated ribosomes.
The IDS allows for profiling of depression, as the questionnaire discriminates directions of the weight, appetite, sleep and psychomotor symptoms, and can be used to determine depression severity.
The high-throughput of next-generation sequencing allows for profiling of transcripts over a wide range of abundance [ 30], providing an accurate picture of differential expression across tissues within an organism.
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Such instruments allow for profiling of risk areas in accordance to different factors such as: temperature, land use and land changes, vector exposure, among others [ 45].
Future work to identify the most efficient routes to ethylene production should include isotope labeling such as C MFA [ 38, 39] to allow for profiling of actual flux maps to complement the in silico modeling approaches.
Additionally, sequencing mRNAs from specific tissues, developmental time points, or experimental conditions allows for rapid profiling of transcript abundance at a global scale [ 1] and analyzing phylogenetically restricted adaptations (e.g., social phenotypes in ants [ 10] and the capsaicinoid pathway in peppers [ 11]).
This study has several strengths; sampling from women in three different African countries; sampling populations of women at different risks for STI and HIV; and a quantitative estimation of abundance for well-known dominant organisms of the vaginal microbiota which allowed for extensive profiling of a relatively large number of samples.
T-RFLP uses fluorescently labeled 5' PCR primers coupled with restriction digests to allow for rapid profiling of unknown microbial communities, providing a less biased picture of microbial diversity than culture-based studies [35].
This array allocates on average four probes per exon for all transcript-confirmed and predicted exons in a mammalian genome, allowing for concurrent profiling of gene expression levels and alternative splicing events.
Because of their small size, miRNAs are more stable than mRNAs; this allows for expression profiling using a variety of tissue sources, including frozen, fixed, and paraffin-embedded tissues, as well as blood and several other biological fluids.
In addition, we show that next-gen TM allows for toxicity profile comparison of compounds for which manual annotation efforts are lacking (triazoles).
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