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The simple recording of cough sound using a microphone and cassette recorder allows for counting of the cough events, however, analysis is very time consuming even with the application of sound activated recording or methods for removing silence [ 7, 8, 13, 14].
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Microfluidic digital PCR provides far better sensitivity for minute quantities of genetic material, but the typical format of this assay does not allow for counting of the absolute number of mRNA transcripts samples taken from single cells.
We explore different circuits that allow for counting and keeping memory of the count after resetting the copy number to 1.
Additionally, it allows for the counting of CTC per milliliter of blood and maintains cell integrity for CTC detection and further characterization.
Culture methods only allow for counting viable cells that are capable of forming colonies on nutrient media, without detecting dead cells, viable but non-culturable bacterial cells (VBNC) and those that require special growth conditions (Oliver 2005; Cerca et al. 2011).
In contrast to RT PCR assays, the CellSearch CTC test allows for the counting of target cells.
A detection threshold of 250 μm, correlated to clinical scorings by experts, was input into a specific software to further allow for automatic counting of the SP density (N/cm) and determination of their respective sizes in mm.
Blood aliquots were also stained with May-Gründ-Giemsa (InstantProv Kit, NewProv, Brazil), allowing for differential counting of basophils, neutrophils, eosinophils and monocytes by light microscopy.
Vials were maintained at 25°C for 18 days to allow for a count of the total number of adult progeny.
As opposed to measuring molecule levels through hybridization to microarrays, this depth of coverage allows for molecule counting such as RNA-derived cDNA (RNAseq) or genomic DNA (re-sequencing) fragments to such a degree that quantitative comparisons can be made between samples.
The Poisson model assumes that crossing over and gene conversion events are independent, whereas the Counting model allows for interference of genetic exchange events.
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