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The versatility of the AuNV design allows for conjugation of longer peptides or mixtures of peptides (i.e., peptides pools) from different tumor antigens onto AuNPs.
Prosthetic groups are bifunctional reagents; one allows for high yield radiohalogenation, and the other allows for conjugation to the biomolecule.
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The cocultures were incubated at 37 °C for 8 h to allow for conjugation and then plated and selected for trans-conjugant cells (recipient strain with the conjugated plasmid) by antibiotics specific for both the recipient strain and transferred plasmid.
In addition, surface modification of NPs to improve biocompatibility and allow for conjugation of biological ligands continues to be actively investigated [19 21].
The OH terminal groups of β-CD were substituted with a thiol group in order to allow for conjugation to the AuNPs.
Methylsulfonyl phenyloxadiazole compounds proved to be highly reactive with Cys residues under a variety of conditions allowing for conjugation with proteins.
The hydrogel coating used in these studies would allow for conjugation of such targeting moieties (proteins, peptides, etc).. To fabricate hydrogel-coated nanoshells, we grew a thin poly NIPAAm- co-AAm) hydrogel coating onto individual gold silica nanoshells using SI-ATRP.
Their increasing use has been attributed to their simple colloidal synthesis, straightforward surface functionalization that allows for their conjugation to a wide range of targeting and/or therapeutic ligands, and their unique optical properties that enable sensitive detection and effective treatment strategies.
This block copolymer self-assembles into nanoparticles with functionalizable maleimide groups on the surface, which allows for further conjugation of targeting peptides.
Similarly, it is important that the molecular structure of the cytotoxin allows for its conjugation to the linker while avoiding immunogenicity, maintaining the internalization rate of the mAb and promoting its anti-tumour effects (i.e., ADCC, CDCC and CDC) [ 46].
Their large surface area also allows for multivalent conjugations, making optimization of the nanoparticles towards in vivo and clinical applications possible.
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