The phrase "allows annealing" is correct and usable in written English.
It can be used in contexts related to materials science, engineering, or processes where annealing is a relevant term, typically referring to the heating and cooling of materials to alter their physical properties.
Example: "The new alloy formulation allows annealing to improve its ductility and strength."
Alternatives: "facilitates annealing" or "enables annealing".
dsRNA was heated to 80°C and cooled slowly to room temperature to allow annealing.
The oligonucleotide mixtures were heated at 95°C for 10 min and then cooled at room temperature to allow annealing.
To prepare probes, 0.5 μM of each oligonucleotide was mixed in annealing buffer (10 mM HEPES-NaOH [pH 7.9], 50 mM KCl, 0.1 mM EDTA), denatured for 2 min at 95°C, and then cooled gradually to allow annealing.
The highly stringent hybridization conditions allow annealing only of the perfectly matching probe, which results in a greater D-to-A distance, thus in an enhanced fluorescence emission of D. Conversely, as the non-matching probes preserve the native coiled conformation, their fluorescence is quenched.
The thermal reaction consisted of a denaturation step at 95°C for 5 seconds, an annealing step at 60°C for 1 min to allow annealing of complementary strands (UOP, APOA5 −1131-S andTTTTCCTCaTGGGGCAAATCTCACTT), and a melting step with a gradual increase in temperature at a rate of 0.1°C/second until 95°C, when fluorescence emissions were read.
The individual components to be assembled are flanked by sites for the restriction endonuclease SfiI that generates non-identical, non-palindromic three base 3′-overhangs designed to allow annealing and ligation of the parts only in the proper order.
The high conductivity is a combined result from the water content and the glycerol in the NFC-G film allowing annealing of the samples across a broader temperature range.
Although the amplification reaction of NASBA is isothermal, a single melting step prior to the amplification reaction is required to allow annealing of the primers to the target, which is not required in LAMP [35, 36].
To create a mutant ssrA gene in which the final two Ala codons of the tag sequence are converted to Asp codons, synthetic complementary oligonucleotides, ssrADD-F and ssrADD-R, were denatured at 96°C for 15 min, cooled slowly to allow annealing and ligated into BamHI/StuI-digested and gel purified pCAN46ΔssrA.
RNA (2 μg) was denatured (65°C, 10 min) in the presence of 1 μl oligo dT17 primer (500 ng μl-1), left at room temperature for 5 min to allow annealing, then kept on ice.
