Exact(9)
To reach a high throughput of candidate gene evaluation in 'Thompson Seedless', a semi-automatic system allowing viable transformation of explants was designed.
After drug incubation of 72 h, 20 µl MTT (4 mg/ml) was added to each well and the plate was further incubated for 4 h, allowing viable cells to develop from the yellow-colored MTT into dark-blue formazan crystals.
After 68 h of incubation, 20 µl of the MTT solution (4 mg/ml) was added to each well, and the plate was further incubated for 4 h at 37°C, allowing viable cells to convert the yellow-colored MTT into dark-blue formazan crystals.
Dormancy levels are higher in the wild rices, allowing viable seeds to persist for years before germination, but these have been reduced in cultivars to give uniform germination.
These lead to changes in SI with increasing diffusion sensitization (b-value), which can be quantified by the apparent diffusion coefficient (ADC), allowing viable tumour to be differentiated from necrosis and inflammation irrespective of the underlying lesion morphology.
The failure of platelets to kill sequestered parasites growing in Duffy-negative red cells may counter intuitively exacerbate this complication by allowing viable parasites to remain within the cerebral vasculature.
Similar(51)
Or ovarian tissue can be removed and frozen in hopes that research now being pursued will one day allow viable eggs to be developed in the laboratory.
Also, green tea, significantly improved the bacterial survival in simulated gastrointestinal environment, and allows viable cells reach a beneficial level of probiotic.
The paint layers were separated from the limestone support and the rendering layers to which they are applied, but it was not possible to individually separate the paint/coating layers themselves as they were too thin to allow viable samples to be taken.
Animals were transferred to fresh plates every day during the progeny production period, and the original plates were left at 20°C for 24 hours to allow viable eggs to hatch and were then stored at 4°C to be scored at the end of the experiment.
However, these prognostic factors do not allow viable prediction of the outcome for the individual patient.
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