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I have also read that microwaving chunks of pineapple for about a minute, allowing to cool and then refrigerating, is said to diminish the acidity and prevents fresh pineapple from making your tongue surface sore.
Paired-end P2 adapter, containing T overhangs, was prepared by combining HPLC purified oligonucleotides P2-PE-FOR and P2-PE-REV (Table 4) in 1X annealing buffer, heating to 95°C and gradually allowing to cool.
Mycobacteria were disrupted by sonication using a bioruptor (Diagenode) for ten cycles (45 sec at high power) allowing to cool in an ice-water bath for 1 min between pulses.
After allowing to cool to room temperature an equal volume of phenol equilibrated with 0.1 M Tris HCl and mixed slowly on a Stuart Rotator SB3 for 10 mins.
It was prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA.
Boil the remaining 1/3 sugar in the last pint of water, allowing to cool before adding to the wine.
Similar(52)
Cover and allow to cool.
Cover, and allow to cool.
Drain and allow to cool.
Remove, and allow to cool.
Drain; allow to cool naturally.
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