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After seeding and growth for a few hours under normoxia allowing to attach to the bottom of flask, A549 cells were cultured in a cell culture hypoxia chamber (0.5%O2/5%CO2/N2 balance) for seven days and then harvested for cell proliferation assay, cell cycle analysis and biological analysis.
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Englishness must never be allowed to attach itself to ethnicity.
When a group takes money from the government, the government is allowed to attach strings.
Because the prison is a national historic landmark, artists were not allowed to attach anything to the walls.
Cells were allowed to attach and grow to ~80 90% confluency (density) for cytotoxicity testing.
Cells were allowed to attach the surface for 24 h prior to treatment.
SH-SY5Y cells were seeded in white 96 well plates and allowed to attach overnight.
The cells were placed in a 96-well plate and allowed to attach for 24 h.
Cells (1 × 105/well) were seeded in 24-well plate and allowed to attach for 24 h.
Cells were allowed to attach overnight and then were treated with different concentrations of compounds.
The cells were seeded into 6-well plates overnight, allowed to attach the bottom.
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CEO of Professional Science Editing for Scientists @ prosciediting.com