The preservative allowed the specimens to be kept for weeks, until they ended up in Dr. Hahn's laboratory.
The 1 week time both for wetting and drying process was selected to allow the specimens to gain and loose sufficient amount of moisture.
The experimental arrangement allowed the specimens to be tested under different input signals and loading conditions, inducing an out-of-plane one-way bending action in the walls.
At the beginning of each test specimens were subjected to 1500 cycles of loading at 25 Hz and 3000 cycles at 50 Hz (with a rest period of 60 s between frequencies) to allow the specimens to stabilise before data collection.
It is suggested that [14, 29] a way of increasing deviation from spherical grains is by increasing the strain rate with the explanation that this allows the specimen to sustain more plastic strain prior to necking.
The system employs an FIB/STEM compatible holder with a specially designed tilt mechanism, which allows the specimen to be tilted through 360° [T. Yaguchi, M. Konno, T. Kamino, T. Hashimoto, T. Ohnishi, K. Umemura, K. Asayama, Microsc. Microanal. 9 (Suppl. 2) (2003) 118; T. Yaguchi, M. Konno, T. Kamino, T. Hashimoto, T. Ohnishi, M. Watanabe, Microsc. Microanal. 10 (Suppl. 2) (2004) 1030].
A hydraulic system that allowed the specimen to be pressurized with PBS solution.
Another physical limitation is imposed on tomography by the current design of specimen holders, which only allow the specimen to be imaged to a tilt angle of ∼70°.
Turn off the oven and allow the specimen to cool to room temperature, then remove the mold from the cast and label it on the opposite side of where cuts will be made for the thin sections (if a label was not added with the specimen).
We are also grateful to the STINASU for allowing the specimen collection in Suriname.
