FM4-64 is a vital dye, allowing labeled zygotes to be followed through development.
Hence, the previous GREAT analysis allows labelling as PS the associated genes.
These values were calculated as the ones that allowed labelling all the important residues of the CSA database in their respective protein's domains.
The near-ubiquitous presence of glucose transporters allows labelling with [F]fluorodeoxyglucose (FDG), but labelling efficiencies are highly variable, the radiolabel is prone to rapid efflux and the short half-life (110 min) of F allows only brief tracking [ 16– 16].
This multi-modal approach allows labelled molecules to be localized in the context of a high-resolution 3-D tomographic reconstruction of the cell.
GCE and LCE metrics allow labeling refinement in either one or both directions, respectively.
Iterative rounds of directed CD tagging using different fluorescent tags allow labeling several pre-selected proteins to study their dynamical relationship in vivo.
The spatial smoothness term is then designed to adaptively encourage label smoothness based on the local cortical folding geometries, i.e., allowing label discontinuity at sulcal bottoms (which often are the boundaries of cytoarchitecturally and functionally distinct regions).
The spatial smoothness term is designed to adaptively encourage label smoothness based on the local folding geometries, i.e., also allowing label discontinuity at sulcal bottoms, where the cytoarchitecturally and functionally distinct cortical regions are often divided.
Lifeact-mEGFP circumvents these artifacts, allowing labeling of actin in living cells.
