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A small brass pedestal was attached to the skull with Charisma (Heraeus Kulzer), using an xyz manipulator, allowing for fixation to a head bar at right angles during training and electrophysiology.
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Polyaxial screws and lamina hooks in conjunction with occipital plating and transitional rods for caudal fixation theoretically allow for fixation points at each vertebra along the posterior aspect of the cervical spine.
Locking plate technology has allowed for fixation of osteopenic bone but the use of unicortical screws in a plating system for the management of a B2-type fracture would unlikely provide an adequately stable construct.
The use of intramedullary rods for fixation of the tibia is attractive since it allows for fixation within the bone sharing the load of walking with the bone and therefore promoting healing, minimizing soft tissue dissection, avoiding the need for supplemental fixation such as a cast and facilitating early weightbearing.
After a 30 min incubation, cells were washed in FACS buffer and centrifuged, and cell pellets were resuspended in BD Cytofix/Cytoperm for 15 minutes to allow for fixation and permeabilization required for subsequent intracellular cytokine staining.
After 72 h on P5, we again allowed for two rounds of 'recovery' in identical salt conditions on plates P6 and P7 (120 g/L), to allow for fixation of beneficial alleles.
The arm was placed in a specifically developed splint to reduce arm movements during the procedure as much as possible and to allow for fixation of the ultrasound transducer.
The proximal humerus internal locking system (Philos®; Synthes®, Oberdorf, Switzerland) consists of a low-profile plate with proximal locking screws allowing for the fixation of fractures previously considered to be inoperable or treatable only with arthroplasty [ 5].
Furthermore, fixations occur more quickly, allowing for more fixations to occur in a given time.
Screw fixation of the C1 lateral mass is a relatively new technique designed to allow for C1/C2 fixation in scenarios where transarticular screw fixation is not safe or possible.
FDR as well as apomixis as a whole are of interest in plant breeding, allowing for polyploidization and fixation of hybrid vigor, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com