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The use of protoplast transfection, coupled with metabolic labelling and pulse-chase experiments, allowed us to very rapidly determine what intracellular location was most suitable for expression of Nef.
Building on the work of other groups [ 48– 51], we utilized a method that allowed us to very reliably measure the volume of the hypothalamus.
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The staining protocol used was developed to allow us to very accurately discriminate cell edges from the background.
"That allows us to very strongly pinch an item, to a much greater degree than a chimp".
The workload is immense, and the rise of social-media tools that allow us to very quickly disseminate information has imposed a burden of alacrity on us all.
This allowed us to use very simple 'hard' rules to annotate clusters with satisfactory performance.
There is very little politics here and very rational management that has allowed us to be very productive".
Although uncommonly used on our ward, the lung assist device allowed us to combine very low tidal volumes (3-6 ml/kg) and high PEEP levels with lower plateau pressures and, thus, the avoided barotraumas reported in these patients.
"But the retrenching has allowed us to get very focused".
Announcing the project "has allowed us to speak very freely to many distributors across many, many platforms".
I loved the innovation in this industry; merging my business skills with colleagues' technical skills allowed us to move very quickly.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com