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The presence of the frt and lox sites allowed us to delete the flanked DNA fragments and to compare the expression of the reporter gene before and after the deletion of the regulatory elements in one genome position.
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Neurod6- Cre expression is evident in developing hippocampal neurons as early as E14 (Goebbels et al., 2006), allowing us to delete Gsk3 in those cells.
If Apple allowed us to actually delete the phone app, I'd bet some of us would do it right away.
This allowed us to precisely identify deleted and shuffled ancestral duplicated genes as the processes driving paleopolyploid genome diploidization.
Introduction of the gene encoding this hybrid histidine protein kinase into cells of S. cerevisiae in which the endogenous Sln1 kinase had been deleted, allowed us to modulate gene expression in the yeast cells with (blue) light.
The [ Dp + Df] progeny allowed us to ask whether the Df deletes any gene with a nucleus-limited function.
The position of known deleted loci allowed us to estimate a minimal size for the deletion of 5.56 Mb.
In this report, we use a mouse model that allows us to conditionally delete the Rb gene and overexpress MYC specifically in the liver.
We have developed a robust protocol which utilises the Ah-cre transgene (possessing an inducible Cyp1a-driven Cre recombinase [ 15]) in combination with loxP flanked Apc alleles [ 16] allowing us to conditionally delete Apc in the adult small intestine [ 11].
The detailed characterization of conserved, deleted, and shuffled duplicates allowed us to unravel chromosome dominance for the investigated grass genomes.
Flickr will also soon roll out functionality that will allow us to restore deleted accounts more easily in the future.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com