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Recent advances in DNA technologies have allowed the sequencing of numerous parasitic species, ranging from viruses to multicellular organisms.
This approach allowed the sequencing of extensive regions of the mtDNA genome within a broad sample group both quickly and in accordance with a limited budget.
A few years later (2002), the Sanger (dideoxy) first generation sequencing, particularly capillary approaches, allowed the sequencing of the first whole human genome, consisting of about 3 billion base pairs and over 20,000 human genes [ 78, 79].
The development of next-generation sequencing technologies has allowed the sequencing of individual genomes to become a reality, documenting the true extent of variation contained within them both with respect to sequence and structural variation.
The development of massively parallel sequencing technologies, coupled with new massively parallel DNA enrichment technologies (genomic capture), has allowed the sequencing of targeted regions of the human genome in rapidly increasing numbers of samples.
DNA Capture Array, exome sequencing, and other target-enrichment strategies for next-generation sequencing have allowed the sequencing of targeted regions in the genome more efficiently and economically (Mamanova et al. 2010; Teer and Mullikin 2010; Mertes et al. 2011), especially with rapidly increasing numbers of samples.
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The genomics revolution of the past decade has allowed the sequences of all human genes to be determined.
The high temporal resolution of the recordings allowed the sequence of spike activations to be identified in many cases, letting us measure directed, rather than undirected, connections.
NGS sequencing has the advantage that it allows the sequencing of single molecule DNA sequences, meaning that the paternal and maternal alleles can be uniquely identified.
Second and third generation sequencing techniques now allow the sequencing of thousands or millions of barcodes, or even entire genomes, in parallel and such high-throughput technologies are available to virtually every laboratory.
We described NaS tool, a hybrid approach allowing the sequencing of microbial genomes using the MinION® device.
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