Currently there are no measurement techniques that allow the localisation and quantification of rotor-based broadband noise sources.
More frequently, pelvic CT and above all MRI allow the localisation of the tumour in the seminal vesicle and a better appreciation of the locoregional extension before surgery.
The cellular localisation of villin in IPEC-J2 cells was visualised by immunofluorescence staining and confocal microscopy, allowing the localisation of immunofluorescense stained structures in the z-dimension of the cell layer (Fig. 9).
Although progress has been made in this area, the development of a robust live cell reporter of expression would allow the localisation of IL-33 during ongoing immune responses.
This allowed the breakpoint localisation to be refined to 1-Mb reginns in chromosome bands 8p21.3 and 12p13.31 (data not shown).
Infrared imaging microscopy allowed for the localisation of incipient brown-rot degradation processes in outer cell wall regions (middle lamellae, primary or outer secondary walls).
PET allows the quantitative localisation of expression of genes coding for membrane receptors and transporters or cellular enzymes.
Whilst confirming that these two types of tumour expressed SRIF receptors, this methodology did not allow the anatomical localisation of receptor expression with respect to the malignant cells or surrounding normal stromal tissues.
Tagging of genes with fluorescent proteins is a rapid and cost-effective alternative to antibodies, which also allows the dynamic localisation of proteins in living cells, and collections of tagged genes based on cDNA constructs have been assembled (Pepperkok and Ellenberg, 2006).
This allowed the algorithm to obtain localisation information at the scale of the insert length variance.
