G1406 is predicted to be in the "switch 1 domain" of the cleft domain, which is thought to be important for the Pol II conformational changes that allow template binding (Gnatt et al. 2001).
Double stranded cDNA was synthesised using the Smart ™ cDNA library construction kit (Clontech UK): single-stranded cDNAs were obtained by reverse transcription, tagged by the SMART IV oligonucleotide, which allow template switching and synthesis of second strand by the reverse transcriptase.
The use of NEMA keys allows templates from different structures to be compared, and allows storage of structures containing a canonical list of templates.
NGS provides powerful, unbiased means for identifying all mutations in an organism in a single sequencing run, but usually requires an a-priori sequenced reference genome to allow template-mediated assembly.
This archetype inclusion mechanism is similar to the COSMIC template-in-template feature, which allows sub-templates to be included in the main template to encourage reuse of templates.
In principle, the Primer ID method, which labels and resequences each individual template, allows quantification of templates and almost complete elimination of experimental substitution and recombination errors (Jabara et al., 2011).
This allows initial template levels to be precisely and accurately quantified.
Concurrently, loop1 between β-strands 3 and 4 in the palm subdomain relocates to allow the template strand to assume its active conformation.
The excellent thermal stability of the crosslinked organic template allowed the high-temperature ALD process to deposit the TiO2 thin film conformally on the nanoline template.
