Exact(3)
A tagged-mutagenesis approach allowed reverse engineering of improved strains, revealing two genes, mutB and cobA, in the primary metabolic branch for methylmalonyl-CoA utilization.
This study has allowed reverse genetics to be used to examine nectary function [13], [16]; however, Arabidopsis nectaries are very small (∼100 microns across and deep) and produce very little nectar [8], [13].
Dimethylation at the second adenosine, A1583 (A1006 in mouse), would therefore be detected only if incomplete methylation at A1584 allowed reverse transcriptase to proceed this far.
Similar(56)
As predicted by the mechanism of action of DCA, this treatment allowed reversing the disturbed lactate and glucose metabolism in septic animals [188].
This design ensured spatial separation of the anion-exchange and the cation-exchange regions, and allowed reversed-phase, anion-exchange and cation-exchange retention mechanisms to function simultaneously.
As predicted by the mechanism of action of DCA, this treatment allowed reversing the disturbed lactate and glucose metabolism in septic animals [ 188].
A major benefit of this novel design is that it allows reverse shaft rotation.
By allowing reverse transcription to proceed, we find that DCs detect HIV-1 in distinct phases, before and after virus integration.
An additional side-effect is that the approach exceeds the requirements of the GNU Lesser General Public License by allowing a software vendor to easily provide a limited amount of source code, rather than potentially releasing source code for large portions of their product suite, or allowing reverse engineering of potentially large portions of their proprietary product.
The other is by mutations that increase phosphorolysis leading to removal of already incorporated chain-terminating inhibitors from the DNA, thereby allowing reverse transcription to continue.
Cell line RNA was used in combination with the stem-loop Multiplex primer pools (Applied Biosystems), allowing reverse transcription of 48 different miRNA in each of 8 RT pools.
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