Exact(3)
Amplification of GAPDH served as an internal positive control and allowed normalisation of the various mRNA levels against the total mRNA content in the samples.
The slow capacitance component measured by this procedure was used as an approximation of the cell surface area and allowed normalisation of current amplitude (i.e. current density).
This allowed normalisation of SLC38A1 and SLC38A2 mRNA expression in each sample to corresponding β-actin mRNA expression, thereby correcting for any differences in reverse transcription efficiency between the samples.
Similar(57)
Gels were stained with Coomassie Brilliant Blue R-250 (Merck and Co., Macquarie Park, Australia) and scanned using the LI-COR Odyssey system (LI-COR Biotechnology, Lincoln, NE, USA) to quantify the total extracted protein and allow normalisation of total protein content in the samples.
Cells were co-transfected with a β-gal-CMV plasmid to allow normalisation of transfection efficiency.
Glyceraldehyde-3-phosphate dehydrogenase and 18S (Applied Biosystems) were used as housekeeping genes to allow normalisation.
This ratio allows normalisation of data and elimination of variability due to cartilage quality, cartilage wet weight, and experimental variation.
Muscle fibres were recovered from the electrode chamber to allow normalisation of oxygen consumption rates to dry muscle mass.
Pre-labelling provides an internal control for cell number by allowing normalisation for the total DNA content of samples.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) pre-designed amplification reagent (Applied Biosystems) was used as a housekeeping gene to allow normalisation.
Antenatal practices and guidelines have changed over the period of this dataset, allowing normalisation of pregnancy when HIV is diagnosed and treated early.
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