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Incubation of protoplasts with pCB1004 and PEG allowed integration of the hygromycin B resistance cassette (under control of the trp C promoter) into the genome of A95.
In addition, the use of a short double stranded DNA oligonucleotide with 3′ overhangs allowed integration of a longer FLAG epitope tag along with a restriction site at rates of up to 50%.
More recent developments of Reverse Genetics and Cell-Free Assembly systems have allowed integration of the accumulated structural and molecular knowledge to be tested at meticulous level, yielding higher insight into basic molecular virology, from which the rational design of safe efficacious vaccines has been possible.
In this example, we developed a modeling framework that allowed integration of the whole biomarker time course and the response.
Nonrepetitive and genetically anchored BAC clones allowed integration of the genetic map with the cytogenetic map by developing definite karyotype markers.
After PCR screening, 60/93 of the tested MiMICs allowed integration of at least one donor in the proper orientation to tag the endogenous gene (Supplementary file 1).
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"They allow integration of wind into the grid.
The case method also allows integration of regulatory and legislative considerations, such as the Sarbanes-Oxley Act of 2002.
This extendible architecture allows integration of future synchrophasor-based applications such as contingency analysis, frequency response, protection and control.
The 'rotamer' approach will allow integration of BCL Conf into respective computational biology programs such as Rosetta.
However, a nodal regulator that allows integration of central and peripheral information and coordinates cardiac rhythmic output has been elusive.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com