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These new guidelines try to clarify some vague wording that allowed for the misreading.
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The high number of CUG codons present in the LacZ gene combined with the different chemical properties of Ser (polar amino acid) and Leu (hydrophobic amino acid) make β-gal a highly sensitive reporter, allowing for monitoring low level misreading activity of the tRNACAGSer.
Though it had been suggested that a person scoring four or more on any one or more of the 12 sets can be categorized as having symptoms, at least two symptoms from a particular category was required in order to receive a syndrome group diagnosis to allow for some degree of misreading or misunderstanding by the respondent [ 13].
Allow for the unexpected.
"She allowed herself to be misread," she said.
The once-brilliant leader is now mocked not just for being clumsy over the budget and allowing Democrats the advantage, but for misreading the popular mood and calling the election wrong.There are curious parallels here with the Democrats.
Even though it is widely used, the cited experimental justification for the translational misreading probability scheme in equation 2 is weak, especially in that translational misreading is more transition-biased in misreading of the second codon position than in the first.
Sadia notched a kill to cut Santa Monica's lead to 20-17, but a return by Thalia Alvarez that the Vaqueros defense misread was allowed to fall right on the back line for a momentum-changing point for the Corsairs.
He chided the Palestinians for misreading the political map in Israel.
Representative Van Hollen called the media out for misreading the report.
This is a much stronger signal than seen for the -1 frame misreading above.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com