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In this framework, the advances performed in silicon surface chemistry allowed for attaching functional groups upon demand [11, 12].
While the ybbR tag allowed for attaching the construct covalently to a coenzyme A surface, the dockerin tag enabled specific non-covalent binding of the construct to a type I cohesin functionalised cantilever tip (see Additional file 4: Figure S4).
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In addition its includes a USB 2.0 port allowing for Network Attached Storage and Print Server functions".
Cells were allowed to attach for 5 h, K02288 added for 30 min and 10 ng/mL BMP9 added for a further 16 h.
Cells were seeded on the microcarrier beads, allowed to attach for 24 hours, and then treated for 48 hours prior to the MR experiment.
compared to cells allowed attaching for 30 to 60 minutes.
They were allowed to attach for 1 hr and the media were changed for either Schneider's or KRB.
Cells were pretreated with IFN- γ for 24 h, subcultured into 96-well plates, and allowed to attach for 24 h.
In detail, cells were seeded in quintuplicate and allowed to attach for 24 hours followed by myostatin exposure under specific treatment conditions for another 24 hours.
The cells were placed in a 96-well plate and allowed to attach for 24 h.
Cells (1 × 105/well) were seeded in 24-well plate and allowed to attach for 24 h.
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