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As Sp2 mRNA is expressed in mouse ES cells (data not shown) the targeting strategy allowed expression of a lacZ-neo fusion gene under the control of the endogenous Sp2 promoter (Figure 2A).
The other parental allele is therefore allowed expression during embryonic development.
Different expression systems with different locations of the recombinant protein were designed and a purification protocol was designed with those which allowed expression of EGF.
The tag allowed expression, purification and easier concentration of the protein, as the CBMCBHI has a molecular weight (MW) of only 3.8 kDa and the CBM-SUMO MW is 19.2 kDa.
His most conspicuous statement of this position comes in his discussions of enthusiasm, where he contended that religious views of all kinds out to be allowed expression and that ridicule of any kind of religion ought also be allowed.
Modified α1-PI gene and its single amino acid variants were cloned in pMAL-c2X vector, which allowed expression of recombinant protein(s) as a fusion of maltose-binding protein (MBP) with factor Xa protease recognition site between the fusion partners.
Similar(29)
"Different societies allow expression at different levels," he says.
Huppert rarely allows expression other than, say, a wince of fastidious disgust at some error of musical interpretation or keyboard address.
Facial mobility allows expression that may be of importance in the behavioral maintenance of interspecific social structure.
As in Wagner's final operas, Beckett's dramas from 1962's Play onward also strip detail to allow expression of the essences of suffering, renunciation and desire themselves.
This bimodal control allows expression and regulation of VDR and its genetic program throughout osteogenesis.
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