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Finally, the use of applied magnetic fields allowed us to activate naive T cell populations, which were otherwise poorly responsive to stimulation.
Eighty-three healthy subjects (42 men, 41 women) participated in this study where we examined pain perception of two tonic heat pain stimulation (thermode) separated by a 2 minute cold pressor test (CPT) (7 °C, 10 °C or 12 °C) which allowed us to activate DNIC.
Using the Gene- Switch line allows us to activate the RNA-hairpin construct after development has finished, minimizing pleiotropic results.
Although the parvalbumin-positive interneurons recruited by this manipulation are typically active during the CA3-dominant phases of theta (Lasztóczi and Klausberger, 2014), causal control allows us to activate them synchronously at arbitrary times during the theta cycle.
Current technology would allow us to express an activated form of the Erbb4 receptor specifically in cardiomyocytes, yet any downstream effect could be due to activation of Nrg1- or non-Nrg1-mediated pathways.
Thus the conditions we used in our analysis allowed us to specifically activate these two stress pathways independently.
The experimental design employed here allowed us to distinguish brainstem areas activated by milk ingestion from those activated by suckling action in rat pups.
First, it allowed us to label precursors that activate the Nkx6-2 locus at different times during development (Fig. 2 D ).
This approach allows us to define the brain areas activated in the different microstates.
The specific labeling of the first wave of activated follicles allowed us to follow their in vivo development to different ages and to determine the time periods that they remain in the mouse ovaries as well as their contributions to the onset of puberty and to fertility.
The transparent body of Daphnia allowed us to determine in which body region activated fluorescent spores attach in the living animals.
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