Seals were sedated with an intramuscular injection of Zoletil (1.2 mg/kg Forte Vet zolazepam/tiletamine; 100 mg/ml, Virbac, Carros Cedex, France) and a central venous catheter inserted into the extradural intravertebral vein, allowing supplementary sedation (0.2 0.3 mg/kg).
Every cell type has a position domain (Ahmed and de Rooij, 2009; Drumond et al., 2011; Hogarth and Griswold, 2010; Nel-Themaat et al., 2011) (newly generated, see the Experiments subsection), within which movement is allowed (supplementary material Table S1).
However, more modern locked nailing implants [29, 33, 36, 37] allow for supplementary syndesmosis screw fixation and are therefore more suited to such injuries.
It is therefore critical to develop additional tests that would provide supplementary information and allow consistent distinguish between idle and aggressive prostate cancer.
In addition, available data from other studies have been taken into consideration to provide supplementary information, thereby allowing EU-wide estimates to be calculated.
One objection to allowing a supplementary tier is that its existence might undermine the basic tier either economically or politically.
Two additional residues, Leu2 and Ala5, are also found in the generously allowed regions (Supplementary Figure S1).
