The presumption is that certain partially extended PCR products would have been quite abundant if they had served as primers for subsequent rounds of PCR to allow their sufficient amplification for detection using cloning with limited numbers of sequenced clones.
The extension time for the iPCR is 3 minutes, while the extension time for the vPCR is adjusted to 14 min. to allow sufficient time for amplification to occur.
Make sure to allow sufficient time for rebuttals.
However, additional repeat testing may be necessary to allow sufficient time for seroconversion [18].
Further examination of polymerases which increase correct sequence amplification but still maintain sufficient amplification for sequencing are required.
The recruitment period is for 12 months to allow sufficient numbers of participants to be recruited.
Two months would allow for sufficient amplification cycles of MVEV activity in the area to result in sentinel chicken seroconversion and then human cases.
The empirical study was conducted in 2003, which allowed sufficient time for trial completion and publication.
This allowed sufficient time for most fractures to heal.
This docking pose allows sufficient space for molecular oxygen.
