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We then placed three females into each tank with the males to allow spawning.
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Adult females and males were bred off- or on-system using breeding traps suspended within 1-L or 3-L tanks, respectively, to allow spawned eggs to settle to the tank bottom.
The game also allowed spawn installations to be made.
Corals were allowed to spawn naturally, following the synchronized mass spawning schedule typical for this and many other coral species [ 37], and then returned to their original locations on the reef.
Individuals from each location were allowed to spawn in the laboratory to produce azooxanthellate juveniles, which were subsequently exposed to six different Symbiodinium types from three phylogenetic clades (A/C2* mixture, C1, C2, C and D).
At first light, the divider was pulled and the adults were allowed to spawn naturally.
All first-generation hatchery fish from the integrated line were allowed to spawn naturally.
The segregated line relied solely on returning hatchery-origin adults for broodstock, and no fish were allowed to spawn naturally.
First-generation hatchery fish began returning as adults in large numbers in 2001 and were allowed to spawn naturally.
If not, should hatchery fish that naturally stray into the new habitat be allowed to spawn there or be culled?
These adults were allowed to spawn freely and, together with their offspring, comprise the Pond data set.
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