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Thereafter, it was transferred to 42°C and incubated for 30 min to allow reverse transcription.
Genome-wide time-series data, in principle, allow reverse engineering of the gene regulatory relationships by studying the temporal patterns of regulators and target genes.
In fact, for each read set we use here, the sum of the sizes of the Mince encoded file and frc, the file which encodes whether or not each read was reverse complemented, is smaller usually by a substantial amount than the encoding size that we would be able to achieve if we did not allow reverse complementing of the reads in the first place.
Many parking lots do not allow reverse parking.
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The dimension reduction comes at the price of loss of information about the association on one of the two dimensions, as the rank-deficiency of M does not allow reversing the reduction applied in (5).
A major benefit of this novel design is that it allows reverse shaft rotation.
A tagged-mutagenesis approach allowed reverse engineering of improved strains, revealing two genes, mutB and cobA, in the primary metabolic branch for methylmalonyl-CoA utilization.
By allowing reverse transcription to proceed, we find that DCs detect HIV-1 in distinct phases, before and after virus integration.
An additional side-effect is that the approach exceeds the requirements of the GNU Lesser General Public License by allowing a software vendor to easily provide a limited amount of source code, rather than potentially releasing source code for large portions of their product suite, or allowing reverse engineering of potentially large portions of their proprietary product.
The other is by mutations that increase phosphorolysis leading to removal of already incorporated chain-terminating inhibitors from the DNA, thereby allowing reverse transcription to continue.
Cell line RNA was used in combination with the stem-loop Multiplex primer pools (Applied Biosystems), allowing reverse transcription of 48 different miRNA in each of 8 RT pools.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com