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Metabolic substrate analogues used in this technique must have high specificity, high signal-to-noise ratio, low cellular toxicity and a low working concentration; furthermore, they should allow labelling in various systems and conditions without interrupting the biological functions of the labelled proteins.
In contrast, radiometalated analogues based on the FFK Sub-KuE -scaffold represent a much more flexible and finely adjustable backbone for the development of KuE-based PSMA inhibitors, that allow labelling with both diagnostic and therapeutic radionuclides, e.g. 68Ga for PET imaging and 177Lu/90Y for therapy after conjugation of DOTA.
The VEGF construct was conjugated to the n-lobe of hnTf to allow labelling with In at a site remote from the VEGF receptor-binding domain.
HeLa cells expressing each of the GPI-anchored proteins were incubated for 90 s at 37°C in the presence of BG-SS-488 to allow labelling and uptake, and extracellular fluorophore was removed by reduction with MESNa.
BN peptides have been coupled to suitable chelators such as diethylenetriaminepentaacetic acid (DTPA), 1,4,7,10-tetraazacyclododecane- N, N′, N″, N′″-tetraacetic acid (DOTA) and acyclic tetraamine (N4, 1,4,8,11-tetraazaundecane functionalised at position 6 for binding to primary amine groups of peptides) to allow labelling with various radionuclides.
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GCE and LCE metrics allow labeling refinement in either one or both directions, respectively.
Iterative rounds of directed CD tagging using different fluorescent tags allow labeling several pre-selected proteins to study their dynamical relationship in vivo.
Hence, the previous GREAT analysis allows labelling as PS the associated genes.
These values were calculated as the ones that allowed labelling all the important residues of the CSA database in their respective protein's domains.
The near-ubiquitous presence of glucose transporters allows labelling with [F]fluorodeoxyglucose (FDG), but labelling efficiencies are highly variable, the radiolabel is prone to rapid efflux and the short half-life (110 min) of F allows only brief tracking [ 16– 16].
This multi-modal approach allows labelled molecules to be localized in the context of a high-resolution 3-D tomographic reconstruction of the cell.
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