For example, expression of W557S by the Act5C driver caused lethality, yet Lamin C protein levels were nearly equal to that of endogenous Lamin C and to that produced by the ey driver, which allowed for viability (Fig. 7).
The alleles responsible for the genetic disorders seen in patients may encode enzymes that retain some residual function, probably more than an active site mutant retains, allowing for viability.
This raises the caveat that our results may not accurately depict the role Pab1 plays in granule formation since suppressors must be present to allow for cell viability.
To allow for larval viability, we again used the conditional Gal4/Gal80TS system to express pro-apoptotic proteins for only 48 72 hr.
We believe that this will allow for enhanced viability in photosensitive tissues as well as reduced photobleaching during imaging set up.
The resulting highly porous and elastic cryogels are biocompatible, and allow for protection of cell viability during compression for injection.
Distribution of synthetic gene circuits among multiple strains allows one to reduce crosstalk among different parts of the overall system and also decrease the energetic burden of the synthetic circuit per cell, which may allow for enhanced functionality and viability of engineered cells.
Following transfection of siRNAs, plates/cells then were treated for 24 h ± paclitaxel (i.e., 3-replicate plates +paclitaxel, 3-replicate plates -paclitaxel) and incubated for an additional 72 h to allow for changes in cell viability.
Clinically established techniques allow for the evaluation of myocardial contractility, viability and perfusion, but do not provide the direct visualisation of transplanted stem cells, therefore their effective presence and viability can be only presumed.
Although OCT allows for imaging of the full vaginal mucosa and submucosa, the resolution does not allow for evaluation of subcellular changes or cell viability assessment.
