Clinically established techniques allow for the evaluation of myocardial contractility, viability and perfusion, but do not provide the direct visualisation of transplanted stem cells, therefore their effective presence and viability can be only presumed.
Syto9/PI labeled biofilms allowed for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell.
To allow for larval viability, we again used the conditional Gal4/Gal80TS system to express pro-apoptotic proteins for only 48 72 hr.
This raises the caveat that our results may not accurately depict the role Pab1 plays in granule formation since suppressors must be present to allow for cell viability.
We believe that this will allow for enhanced viability in photosensitive tissues as well as reduced photobleaching during imaging set up.
For example, the high growth rate of bacteria and yeast reasons the requirement of assays which allow for viability tests covering more than three orders of magnitude, which excludes classical colorimetric assays.
The performed examinations did not allow estimation of the viability of the oocysts and, consequently, the infectious ability for humans was not determined.
We developed a method for maintaining embryo viability after yolk removal, which allowed for the first time a clear visualization of developing late endocrine cells in living embryos.
To allow accurate evaluation for the viability of the limb, we decided to perform a second-look operation after 48 h.
Distribution of synthetic gene circuits among multiple strains allows one to reduce crosstalk among different parts of the overall system and also decrease the energetic burden of the synthetic circuit per cell, which may allow for enhanced functionality and viability of engineered cells.
The resulting highly porous and elastic cryogels are biocompatible, and allow for protection of cell viability during compression for injection.
