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A standard curve was prepared utilizing purified recombinant EGFR protein to allow for quantitation of EGFR.
The d.n.FoxO is fused to a DsRed protein tag to allow for quantitation of the ectopic protein and has been used and described by our lab previously [ 16, 19].
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This extraction technique allowed for quantitation of milataxel and its major metabolite s-lactate (M-10) from tumors and brain tissue samples using HPLC coupled with electro-spray ionization mass spectrometry (HPLC-ESI-MS).
Due to the low number of cells available from each isolation, we performed 3D Telo-FISF analysis, allowing for quantitation of telomere length of each individual cell [30].
Dramatically, p-IRE1α exhibited one discrete slow migrating band in the Phos-tag gels, a feature that allows for quantitation of the percent of p-IRE1α (see below).
This assay allows for quantitation of cell survival, and because the cells were directly visualized in the feces (frass), ensures that they have passed through the gut rather than having been transferred to the cover slip from the exterior of the fly.
TLDA allows for quantitation of 335 mouse and 226 rat miRNAs based on Sanger mirBase v. 10 annotations.
The small number of spinal motor neurons in SMND patients did not allow for formal quantitation of AMG staining.
In order to allow for relative quantitation of gene expression, we used the reference genes 18S and GADPH.
While the MDAP platform is similar in design to SBE assays, its improved sensitivity and specificity allow for accurate quantitation of low frequency polymorphisms within a mixed population.
The amplified single-stranded cDNA was purified using the Zymo Research Clean and Concentrator™-25 Kit to allow for accurate quantitation of the cDNA and to ensure optimal performance during the fragmentation and labeling processes.
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