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Unlabeled reporter peptide R9L was included in each assay as competitor to allow for normalization of data between different assays.
In both cases, subtracted cDNAs were subjected to two PCR cycles to allow for normalization of cDNA populations.
Additionally, expression of the 18S ribosomal subunit was also assessed to allow for normalization of total RNA levels between samples using the 2−ΔΔCT method [38].
The gels were subsequently stained with Deep Purple total protein stain to allow for normalization of the Pro-Q Diamond signal according to total MYBP-C content.
At least one hour passed after the insertion of the probe and the start of the sampling to allow for normalization of changes due to probe insertion.
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Even though 60-fold more vge/cell was utilized with HPV18 compared to HPV45, this allowed for normalization of RT-qPCR data, allowing amplification of nucleic acid at similar Ct values (data not shown).
Even though 10-fold more vge/cell was used with HPV31 compared to HPV16, this allowed for normalization of RT-qPCR data, allowing amplification of nucleic acid at similar Ct values (data not shown).
We used cell-line controls to span the dynamic range of the measurements, allowing for normalization of the data across multiple batches and ensuring our ability to compare different batches.
This latter antibody, which detects BTPC polypeptides independent of their phosphorylation status, allowed for normalization of total BTPC on immunoblots.
Primers that specifically amplify either cDNA or gDNA were then used for qRT-PCR, allowing for normalization of gene expression (cDNA amplification) to genome copy number (gDNA amplification).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com