Sentence examples for allow fluorescent detection of from inspiring English sources
Microsatellite regions were PCR-amplified using a tailed primer approach [ 66] to allow fluorescent detection of the PCR products on a LiCor (Lincoln, Nebraska) Automated Sequencer (DNA Analyzer 4300).
While the fluorophore may allow fluorescent detection of binding of the compound to the apoptotic cells, F-labeled NST 732 may allow detection of apoptosis in the clinical set-up, using PET.
We modified Lifeact at the C-terminal by adding a Lys residue labelled with carboxyfluorescein (FAM) so as to allow fluorescent detection of actin staining; at the N-terminal we added a Cys residue for disulfide bond formation with the carrier.
Annexin allows fluorescent detection of Annexin V bound to apoptotic cells.
Cells were then incubated with Alexa-conjugated fluorescent secondary antibodies (Invitrogen) for 1 hour in the absence of light at room temperature to allow the fluorescent detection of desired proteins.
Fluorotect Green-Lys, is a BIODPY labeled lysine that allows the addition of a fluorescent amino acid to any newly synthesized peptide (unlabeled lysine is also included in the reaction mixture) allowing for fluorescent detection of such peptides.
Its unique biphasic Zn II -binding behavior could be attributed to the presence of two distinct Zn II -binding sites and allowed ratiometric fluorescent detection of Zn(II) over a concentration range from 10 nM to 1 mM.
Amplified Fragment Length Polymorphism (AFLP) banding patterns were generated using a modified version of the protocol described in [30], that allowed for fluorescent detection of labeled AFLP's.
Amplified fragment length polymorphism manipulations were performed essentially as described by Vos et al. (1995), using a modification that allowed for fluorescent detection of the amplified fragments.
To monitor growth of P. putida under environmental conditions, we constructed plasmid pBAM1- RparsM-gfp, in which green fluorescent protein was fused to the C-termus of RparsM in place of the six histidine tag, allowing direct fluorescent detection of hybrid protein production rather than just mRNA synthesis.
We used a fluorescently labelled primer to allow electrophoresis and subsequent fluorescent detection of products using an Applied Biosystems ABI 3130XL capillary electrophoresis machine.
