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KABUL, Afghanistan, Oct.13 Â Two international election officials, appointed by the United Nations to examine complaints from presidential candidates of irregularities in Saturday's election, said they expected to finish a first review of the charges by this evening, to allow counting of the votes to go ahead.
To allow counting of the plaques, the cell monolayer was stained with crystal violet after fixing the cells with 10% paraformaldehyde.
The fluorescein-12dUTP-labeled DNA was directly visualized by fluorescence microscopy with excitation at 520 ± 20 nm, to allow counting of the proportion of apoptotic cells (nuclei with green fluorescence).
The papillary processes on each anal fin ray were counted with only the photographs taken at DAF 124, because fish at DAF 104 and younger were too small to allow counting of the papillary processes based on the pictures.
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But it was a very fluid status quo, because the court also expressly allowed counting of the absentee ballots and the hand recounts to continue.
For each species, the ESTs obtained were assembled with the ORF sequences using GAP4 from the Staden package [ 45], which allowed counting of the number of identical ESTs associated with an ORF.
Stable isotope labeling methods allow counting of C and N per formula unit and can lead to identification of the chemical formula without reliance on the natural isotopic abundance patterns and without using a restricted chemical formula generator.
After 8 days in culture, flasks were fixed in 0.5% glutaraldehyde in PBS, clones were photographed and the flasks were then stained with haematoxylin to allow counting of colony numbers.
A limitation of this approach was the fact that in the anteroventral periventricular nucleus (AVPVn) staining was too dense to allow counting of individual cells.
A 10 μl aliquot of the cell suspension was mixed with 10 μl of Trypan blue (Gibco-Life Technologies, NY, USA) solution to allow counting of nucleated cells.
F4/80 staining allowed counting of macrophages in the entire cross-sections.
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