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Overall, these observations indicate that the structured parts of Mad1 are required to bring the Mad1 Mad2 complex to kinetochores to allow checkpoint signalling, potentially through an interaction between Mad1 and Bub1.
Current understanding of the role of mitotic kinases in checkpoint recovery can be summarized as: 1) these kinases are inhibited by the DNA damage checkpoint to allow checkpoint activation and prevent mitosis; and 2) reactivation of these kinases deactivates checkpoint signaling and leads to cell recovery.
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Composite applications using in-node shared memory can reduce the amount of data to be communicated between nodes, allowing checkpointing and data reduction or analytics to be performed locally and in parallel.
Collectively, the studies summarized above revealed an essential role of Plk1 in checkpoint recovery by directly targeting multiple DNA damage checkpoint factors, and thereby allowing checkpoint-deactivation and cell cycle reentry.
Furthermore, the dbf4-Δ71-221 protein that cannot interact with Rad53 (Chen et al., 2013) also did not allow for checkpoint bypass.
Curiously, the kinetics involved during abscission are considerably slower than what has been observed in retroviral budding, potentially to allow for checkpoints that ensure the fidelity of cytokinesis.
Transcriptional repression is suspected to be an important mechanism to keep stress responses under tight control [ 43- 45]; early response to NPs might involve preventive repression of gene expression to allow genetic checkpoints to be readjusted before further responses are triggered.
As RAxML-Light, ExaML also allows for checkpointing and subsequently re-starting tree searches from light-weight binary checkpoints.
When the Afghan Army arrived, soldiers pushed the Taliban back, allowing the checkpoints to be re-established.
Access to the Kurdish front was closely controlled, with only a small selection of media allowed through checkpoints as part of a carefully marshaled convoy and instructed not to question individual peshmerga.
While Donetsk markets are still reasonably well stocked – traders say they offer fruit and other foodstuffs to both Ukrainian and rebel soldiers as bribes to allow them through checkpoints – there are few locals with the funds to buy them.
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