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Thus, it is possible that Lyme disease borreliae capture CFHRs or lipoprotein particles to allow adherence to host epithelial cells and tissues, as recently described for CFH-coated S. pneumoniae [59].
After incubation, the cells were washed twice by centrifugation and resuspension in AIP; ∼4·106 cells were then transferred to a chamber with glass bottom coated with poly-L-lysine (#1.0, MatTek Corp., Ashland, MA, USA) and further incubated at 37°C for 60 minutes to allow adherence to the coverslip.
This CNS-active molecule was considered suitable for extensive modification and was of sufficiently low molecular weight to allow adherence to Lipinski's rules, even following considerable alteration.
Other cell wall proteins thus could interact with FN to facilitate complex formation and, in this way, allow adherence to epithelial cells.
Such guidelines may well help to improve care, because they specify optimal care for care providers and allow adherence to be monitored.
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The in vitro cell binding was assessed by growing B16F1 cells in culture flasks to 80 90% confluence, trypsinizing, washing in PBS (pH 7.4), aliquoting into 12-well plates and allowing adherence to the wells overnight.
The products differ from conventional cosmetics in that they are waterproof and opaque, allowing adherence to textured skin, including scarred or diseased areas.
These fragments were placed on the surface of 100 mm Petri dishes for 40 50 minutes to allow adherence of the tissue to the culture surface.
Washed fibers were plated onto a 35-mm culture plate that had been previously coated with a collagen matrix (Matrigel, BD Biosciences) and incubated for 18 24 h covered with culture medium at 37°C in 5% CO2 in a humidified atmosphere to allow adherence of the fibers to Matrigel.
These experiments were performed in the presence of endothelial cells, to allow adherence of the RGD-equipped complexes to αvβ3 integrin expressing cell layers.
MSCs (2×10 cells) were seeded on to a Matrigel™-coated coverslip and incubated for 45 min at 37 °C to allow adherence.
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