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Allocation of the sequence was concealed until after the interventions were assigned.
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For sample-wise allocation of the sequencing data, the raw reads from each lane of flow cell were demultiplexed using the respective index sequence.
After phylogenetic allocation of the sequences down to the family or genus level, the sequences belonging to different phylogenetic groups were added together and depicted as a percentage of the total sequencing reads obtained from each sample.
The random allocation of the treatment sequence was based on a computer-generated random number sequence.
In the first iteration, one seeks a best allocation of the novel sequence to a node of the classification tree T that explains most of the (weighted) mutations relative to rCRS and subsequently searches for a closest representative beyond this ancestral sequence in the total database (including the new data under examination).
After this order is established the allocation of the random sequence will be performed by the researcher.
Until the opening of the seal of the envelope, both the supervisor/researcher and the mothers were kept blind to the allocation of the intervention sequences.
Allocation of the random generated sequence will be by consecutively numbered envelopes.
5 For each patient, baseline, year 1 and year 2 radiographs were all re-read at year 2 by two independent expert readers blinded to the original treatment allocation and the sequence of films.
Beyond aggregated indicators such as trips, tours or time-budget allocation, the sequence of activities performed in the course of one day is suitable for reflecting the space-time organisation of activities.
The primary investigator (JB) will implement the allocation and hold a record of the sequence.
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