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Barcodes are distinct between sample libraries and allow pooling for sequencing followed by allocation of reads back to individual samples after sequencing by analysis of the sequenced barcode.
We also investigated the diversity of start points in transcripts with higher abundance and found an interweaving, balanced allocation of reads (exemplified in Additonal file 1, figure S2) in our dataset.
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RSEM executes an EM algorithm to compute a proportionate allocation of the reads with multiple alignments.
The jury asked to have Mr. Quattrone's testimony about his role in the allocation of stock offerings read to them.
All multiplexing strategies are based on small DNA fragments termed 'barcodes' that are attached to the fragments to be sequenced, and thereby enables the allocation of every single read to each sample.
Within one week, two trained reference microscopists who were blinded to each other's results and the allocation of the patient double read the slides using light microscopy.
For more, along with a detailed chart of each state's allocation, read this post on Inside Higher Ed.
A logical start to achieving compliance is provision of information and education about IC and allocation of time for staff to read and learn about the procedures [ 16].
For sample-wise allocation of the sequencing data, the raw reads from each lane of flow cell were demultiplexed using the respective index sequence.
In these rare cases manual editing was needed by incorrect allocation of a low number of sequence reads of co-amplified pseudogenes (DRB2, DRB6, DRB7 and DRB9) or PCR artefacts formed through in vitro recombination between DRB1 and DRB3/4/5.
Allocations will read either: '1: Control, 2: Intervention', or '1: Intervention, 2: Control'.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com